National Magnetic Resonance Facility at Madison
From BTRR
Overview
The National Magnetic Resonance Facility at Madison (NMRFAM) develops multinuclear, multidimensional nuclear magnetic resonance (NMR) approaches to solution-state studies of biological macromolecules, such as proteins, nucleic acids, carbohydrates, and lipids. Related collaborative projects converge on structure-function investigations of proteins and nucleic acids, structural genomics, and metabolomics. The resource has considerable experience in labeling proteins and RNA with stable isotopes and in studying chemical and dynamic properties of biomolecules, such as binding equilibria, conformational equilibria, internal mobility, and protonation steps. Data collection for oxygen-sensitive samples and proteins containing paramagnetic centers is a specialty. NMRFAM develops methods for efficient NMR data collection, processing, structure determination, validation, and data deposition. Computational resources include not only hardware and software but also expertise in ab initio calculations of chemical shifts and J-couplings from structural modeling.
Current Research
Fast data collection and automated data analysis. Technology for larger proteins and complexes. Metal-containing (paramagnetic) proteins. Dynamics of macromolecules. Structure-function investigation of RNA molecules and their complexes with metal ions and proteins.
