National Resource for Biomedical Accelerator Mass Spectrometry
From BTRR
Overview
The National Resource for Biomedical Accelerator Mass Spectrometry was established to make accelerator mass spectrometry (AMS) available to biomedical investigators who have a need for accurately measuring very low levels of carbon-14 in their research. The resource has three major functions: to develop and refine methods and instrumentation for the use of AMS in biomedical research; to provide biological researchers with access to AMS through either collaborative or service arrangements; and to demonstrate new applications for AMS and provide outreach activities for educating researchers and students on the use of biological AMS.
Current Research
Building on the initial development of sample preparation methodology, the current focus of the resource is on expanding capabilities by developing methods to: 1) study biochemical pathways and cellular processes at the level of the single cell; 2) quantify endogenous processes in higher organisms; and 3) develop a routine sample preparation methodology for tritium AMS.
To these ends, collaborative studies have been established to: 1) quantify metabolites in single yeast cells; 2) quantitative biomarkers of DNA and protein oxidation; and 3) facilitate the measurement of tritium or dual-isotope labeled biological samples through sample-spectrometer interface development.
The ongoing effort continues the investigation of methods to study protein biochemistry and cell biology and develop isotope derivatization approaches. The resource aims to analyze 14C and/or 3H in tissues, macromolecules, and metabolite extracts. Applications under investigation include studies to determine the effects of toxicants in humans; to identify protein targets for drugs, nutrients, and toxicants; and to determine the pharmacokinetics of micronutrients.
